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KMID : 0358320070480040444
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Korean Journal of Urology
2007 Volume.48 No. 4 p.444 ~ p.451
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The Histological Changes and Expression of Hypoxia Inducible Factor-1α and Vascular Endothelial Growth Factor according to the Differential Renal Function during Total Ureteral Obstruction in the Rabbit Model
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Shim Ki-Sik
Lee Sang-Don
Lee Kyong-Mi
Kim In-Ju
Kim In-Joo
Kim Jee-Yeon
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Abstract
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Purpose: The renal histological and hemodynamic changes and the expressions of hypoxia inducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF) were evaluated according to the differential renal function(DRF) during total ureteral obstruction(TUO) in a rabbit model.
Materials & Methods: In forty-nine control(5) and 16 experimental rabbits(16 in TUO 3 days, 13 in TUO 7 days and 15 in TUO 14 days), the renal blood flow(RBF) and 99mTc-DTPA renal scan were measured both before and after TUO. The cut-off of the DRF group was 40%. The histological changes and expressions of HIF-1α and VEGF were evaluated using H&E and immunohistochemical stain, respectively.
Results: The entire control group demonstrated more than 40% DRF. Contrary to the control group, the DRF was less than 40% in 4(25%), 7 (53%) and 6 rabbits(40%) in TUO 3, 7 and 14 day groups, respectively. The postobstructive compared to preobstructive RBF was decreased in each group. The RBF was more decreased in the lower than the higher DRF group(more than 40%) in all of the experimental groups. Abnormal histological changes were more prominent in the experimental groups, and increased with the obstruction time. However, there was no difference in relation to the DRF. The expressions of HIF-1α and VEGF were more prominent in the experimental and lower DRF groups.
Conclusion: During acute TUO, the decreased RBF and hypoxia may play a role in preservation of the DRF. (Korean J Urol 2007;48:444-451)
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KEYWORD
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Ureteral obstruction, Kidney function, Hypoxia-inducible factor 1, Vascular endothelial growth factor, Histology
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